大鼠8羟基脱氧鸟苷ELISA试剂盒

产品名称：人8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒

英文名称：Human 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

产品分类：人代谢相关酶联免疫吸附测定试剂盒[ELISA Kit for Human metabolism]

检验方法：ELISA

包 装：96T

人8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗8-OHdG抗体的微孔中依次加入标本或标准品、生物素化的抗8-OHdG抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成**终的黄色。颜色的深浅和样品中的8-OHdG呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

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